How to Use a Pipettor: Your Easy Guide

Pipettors are hand-operated tools that are used to transfer small volumes of liquids or fluids from one vessel to another. Pipettors come in different styles and sizes. However, they all function under the same principle: applying negative pressure on a fluid-filled tip will cause the fluid to be drawn up into it.

Pipettors are typically used by lab technicians and scientists to transfer reagents from one place to the next. They are also used to dispense accurate amounts of liquid onto a reaction plate. The first glass pipettes were developed in the 17th century. Since then, they have been considered an important part of laboratories worldwide.

How to Use a Pipettor

When using a pipettor, you need to first select the appropriate tip and insert it into the plunger’s end. From there, you can draw up the desired volume of liquid. You can do this by pulling back on the plunger until it reaches the level you want.

It is crucial that you set the right volume on the dial before you start a new task or experiment since the plunger should not go below or above that level. Once the tip has been filled to the amount desired, it can now be released.

To release, push the plunger down and press it firmly against a flat surface. Ensure your hands are clean when using a pipettor. It is also crucial that you don’t touch the tip of the pipettor. To ensure you won’t cause cross-contamination or contaminate the samples, the devices should be used with disposable tips. They are fitted over the plunger similar to a collar.

When dispensing fluid onto the experiment plate, you need to first make sure your hands are clean and the tip from the plunger has been removed. It is also important that you retain a firm grip throughout the transfer process.